By María Isabel Pividori, Salvador Alegret (auth.), Christine Wittmann (eds.)
M.I. Pividori, S. Alegret: DNA Adsorption on Carbonaceous fabrics .-
F. Luderer, U. Walschus: Immobilization of Oligonucleotides for Biochemical Sensing by means of Self-Assembled Monolayers: Thiol-Organic Bonding on Gold and Silanization on Silica Surfaces.-
Y. Okahata, T. Kawasaki: training and Electron Conductivity of DNA-Aligned solid and LB movies from DNA-Lipid Complexes.-
A. del Campo, I.J. Bruce: Substrate Patterning and lively options for DNA Chip Fabrication.-
D.V. Nicolau, P.D. Sawant: Scanning Probe Microscopy reviews of Surface-Immobilised DNA/Oligonucleotide Molecules.-
A. Guiseppi-Elie, L. Lingerfelt: Impedimetric Detection of DNA Hybridization: in the direction of close to sufferer DNA Diagnostics
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Additional info for Immobilisation of DNA on Chips I
With the modiﬁcation of dsDNA, the CP surface turned from poor to high hydrophilicity and the hydrophilic surface could survive the thorough washing with water, which indicated the tight combination of DNA on the electrode surface . A chitosan-modiﬁed CP (ChiCP) material was prepared for the electrostatic adsorption of dsDNA, ssDNA and ODNs . The immobilized ODN could selectively hybridize with the target DNA to form a hybrid on the ChiCP surface. 2 DNA Modifying Carbon Pastes as an Additive Additives such as polyethylene glycol, cationic antibiotics, polymers, small uncharged molecules, and negatively charged proteins have been used extensively in order to avoid the denaturing of enzymes or to improve the sensitivity and operational stability of biosensors.
Faster molecular movement and changes in structure could facilitate the adsorption. More electrochemically active sites of the nucleic acid molecules could come into close contact with the electrode surface, thus increasing the signal. I. Pividori · S. Alegret molecules such as dsDNA, this effect has proved to be relatively strong compared to that in less complex ones such as tRNA . 5). 3 V was also demonstrated to be successful for immobilizing dsDNA on CP . 1 Surface-Modified Carbon Pastes CP was surface-modiﬁed with cetyltrimethyl ammonium bromide (CTAB) by dry adsorption (CTAB/CP) .
Alegret 111. Bucková M, Labuda J, Sandula J, Krizková L, Stepánek I, Duracková Z (2002) Talanta 56:939 112. Labuda J, Bucková M, Heilerová L, Caniová-Ziaková A, Brandsteterová E, Mattusch J, Wennrich R (2002) Sensors 2:1 113. Labuda J, Bucková M, Jantová S, Stepánek I, Surugiu I, Danielsson B, Mascini M (2000) Fresenius J Anal Chem 367:364 114. Wang J, Kawde AN, Sahlin E (2000) Analyst 125:5 115. Wang J, Kawde AN, Erdem A, Salazar M (2001) Analyst 126:2020 116. Wang J, Kawde AN (2001) Anal Chim Acta 431:219 117.
Immobilisation of DNA on Chips I by María Isabel Pividori, Salvador Alegret (auth.), Christine Wittmann (eds.)