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By James F. Rusling

ISBN-10: 0824708997

ISBN-13: 9780824708993

Examines movies of biomolecules which may supply stable surfaces for catalyzing enzyme reactions, serve in biosensors and as biorecognition components, mediate nanoparticle formation, and supply a easy for basic stories and functions in biomedical units.

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Additional resources for Biomolecular Films Design Function and Applications

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Mb was also studied in composite ¢lms of DDAþ and polyacrylate À (PA ) [172]. Electrochemical results and a Tc of 18 C compared of 11C for DDABÀMb ¢lms suggest slightly less £uidity in DDAÀPAÀMb ¢lms compared to DDABÀMb. Electrochemical sensors based on antigenÀantibody interactions show great promise for biomedical and biohazard detection. In a study optimizing surfactantÀpolyion ¢lms for such uses, electrical impedance was used to show that molecular organization of ¢lms of dioctadecyldimethylammonium bromide with poly(styrenesulfonate) (PSS) or Na¢on on pyrolytic graphite electrodes was improved by annealing in water [173].

An example of a ¢t is shown in Fig. 7. The mean values of reorganization energies for reduced and oxidized forms of Mb, respectively, found by this method were lRED ¼ 0:41 eV and lOX ¼ 0:21 eV. FIG. 7 Experimental current (s)with model background current subtracted resulting from regression analysis of SWV data at 200-Hz frequency, 160-mV pulse height shown with the best-fit line from the Marcus model. The Marcus model was fitted to raw SWV data including background, and the model background was then subtracted.

Films of Mb and the lipid DMPC are used to illustrate CV behavior for the catalytic reactions. In the absence of reactant trichloroacetic acid (TCA), a reversible thin-¢lm CV is found for the heme FeIII /FeII couple of myoglobin Copyright 2003 by Marcel Dekker, Inc. All Rights Reserved. FIG. 5. Numbers on curves show millimolar TCA concentration in the buffer; dashed curve to the far right for TCA on a DMPC-coated electrode without Mb. (Adapted with permission from Ref. 53. ) (Fig. 9). Additons of TCA cause increases in the FeIII reduction peak with increasing concentration and decreases in the FeII oxidation peak.

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Biomolecular Films Design Function and Applications by James F. Rusling

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