By Jitra Waikagul
Approaches to investigate at the Systematics of Fish-Borne Trematodes is a concise consultant for systematic stories of the superiority of fish-borne trematodes either within the endemic components and experimental laboratories. It contains easy methods to establish species of fish-borne trematodes to reinforce the precision of analysis stories according to the metacercarial stage.
Misidentification of trematode species is a typical incidence while researchers are new to the sphere and feature no counsel. Consequentially, occasionally courses document faulty incidence premiums of those parasites. This compact advisor supplies transparent course on:
- Collection of parasites within the ultimate hosts
- Collection of cercaria from snail first intermediate hosts
- Collection of metacercaria from fish hosts
- Molecular id of parasites
- Systematics of fish-borne trematodes
- Provides examine guidance and protocols for learning systematics of fish-borne trematodes utilizing either morphological and molecular data
- Presents keys to let identity of metacercariae of fish-borne trematodes within the better Mekong subregion
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Extra resources for Approaches to Research on the Systematics of Fish-Borne Trematodes
Take a photograph of fish and identify fish with key. 2. Measure and record the number of fish in each species, the date, and the locality of the fish examined. 3. Examine the pectoral fin, tail fin, body muscle, and internal organs of the fish with a stereomicroscope. 4. Separate the metacercaria from the fish using dissecting needles, and pipette it into a block glass. 5. Identify the metacercaria by stereomicroscope if possible; if that fails, examine it under a light microscope. 6. Keep the metacercariae in vials with normal saline solution for further study, with 70% alcohol for molecular study, and in 5% formalin for staining.
Separate the metacercaria from the fish using dissecting needles, and pipette it into a block glass. 5. Identify the metacercaria by stereomicroscope if possible; if that fails, examine it under a light microscope. 6. Keep the metacercariae in vials with normal saline solution for further study, with 70% alcohol for molecular study, and in 5% formalin for staining. 7. Label with the date, the species of metacercariae and fish (coding if not known), the locality where the fish was found, and the formula of the solution in the vial.
Le TH, De VN, Blair D, et al. Clonorchis sinensis and Opisthorchis viverrini: development of a mitochondrial-based multiplex PCR for their identification and discrimination. Exp Parasitol 2006;112:109À14. 15. Sato M, Pongvongsa T, Sanguankiat S, et al. Copro-DNA diagnosis of Opisthorchis viverrini and Haplorchis taichui infection in an endemic area of LAO PDR. Southeast Asian J Trop Med Public Health 2010;41:28À35. 16. Stensvold CR, Saijuntha W, Sithithaworn P, et al. Evaluation of PCR based coprodiagnosis of human opisthorchiasis.
Approaches to Research on the Systematics of Fish-Borne Trematodes by Jitra Waikagul