By Ben Dawes (ed.)
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Extra resources for Advances in Parasitology, Vol. 3
H . R. , in EATRO, 1962-63). These examples indicate the value of the method of infectivity titration in yielding quantitative information on trypanosome potentiality independent of trypanosome numbers. It may be expected also to be a more sensitive tool than counts of the numbers of organisms. Infectivity measurement should be applicable down to levels of a few infective organisms per ml of suspension. Trypanosomes can only be easily and accurately counted when they are fairly concentrated, say more than antilog 5 organisms per ml.
1937) did allow a substitute meal and their results were similar. Wijers considers that the effect is likely to be related to the development of the peritrophic membrane. 2. Development in the Haemocoele Some experiments on the development of T. brucei subgroup trypanosomes in the haemocoele of Glossina after artificial parenteral inoculation have been reported (Webber, in EATRO, 1961). In nearly all flies inoculated with between 70 and 230 x lo3 organisms, further development took place in the haemocoele.
1 and 0%. Wijers did not allow any substitute meal to the flies which were not allowed to feed on the infected host on the 1st and 2nd days and so his result might have been related to some starvation effect. However, van Hoof et al. (1937) did allow a substitute meal and their results were similar. Wijers considers that the effect is likely to be related to the development of the peritrophic membrane. 2. Development in the Haemocoele Some experiments on the development of T. brucei subgroup trypanosomes in the haemocoele of Glossina after artificial parenteral inoculation have been reported (Webber, in EATRO, 1961).
Advances in Parasitology, Vol. 3 by Ben Dawes (ed.)