By L. Scott Cram (auth.), R. C. Sobti, Awtar Krishan (eds.)
Flow cytometry has speedily developed right into a method for swift research of DNA content material, mobile marker expression and digital sorting of cells of curiosity for extra investigations. stream cytometers are being generally used for tracking of mobile DNA content material, phenotype expression, drug shipping, calcium flux, proliferation and apoptosis. Phenotypic research of marker expression in leukemic cells has develop into a big software for diagnostic and healing tracking of sufferers. contemporary stories have explored using movement cytometry for tracking hormone receptor expression in human strong tumors and for reports in human genomics. Contributions within the present quantity are in line with shows made on the First Indo-US workshop on movement Cytometry during which specialists from united states, united kingdom and India mentioned functions of move cytometry in organic and scientific study. This ebook should be of curiosity to submit graduates and researchers within the fields of pathology, cytology, telephone biology and molecular biology.
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Extra resources for Advanced Flow Cytometry: Applications in Biological Research
In particular the identification of translocations by the application of chromosome specific probes derived from sorted chromosomes. This is a particularly powerful technique that has 13 if\ .... • ~ 9 · 12 - der 15 I 80 Q) ~ 70 g 60 2! u:: 15 22 ... /~ t22 ""'1 9 DebriS t~ 15 ~ derlS 1(15;22) Chromomycm A3 Fluorescence Inlens ty Figure 7. Flow karyotype of a patient with a translocation, t(15 ; 22). 22 32 seen limited application due to the commitment of resources necessary to make it a simple routine clinical technique.
Kakino S, Sasaki K, Kurose A, Ito H (1996). Intracellular localization of cyclin B 1 during the cell cycle in glioma cells. Cytometry 24: 49-54. 3. Kallioniemi O-P, Wagner U, Kononen J, Sauter G (2001). Tissue micro array technology for highthroughput molecular profiling of cancer. Human Mol Genet 10: 657-662. 4. Kamentsky LA, Burger DE, Gershman RJ, Kamentsky LD, Luther E (1997). Slide-based laser scanning cytometry. Acta Cytol 41: 123-143. 5. Kawasaki M, Sasaki K, Satoh T, Kurose A, Kamada T, Furuya T, Murakami T, Todoroki T (1997).
A good figure of merit for high purity sorting on a conventional flow sorter is about 106 chromosomes in 6 hours. 4 x 107/ml. High speed sorting of chromosomes can increase this rate by roughly a factor of 5 without seriously diminishing resolution or recovery. At faster analysis rates resolution is seriously degraded, as is purity. 4. Cytometric technologies for the human genome program Figure 6. Bivariate flow karyotype of a mouse/human somatic cell hybrid. Chromosome sorting and analysis played a major role in the early stages of the human genome program.
Advanced Flow Cytometry: Applications in Biological Research by L. Scott Cram (auth.), R. C. Sobti, Awtar Krishan (eds.)